Volume No. :   2

Issue No. :  4

Year :  2010

ISSN Print :  0975-4393

ISSN Online :  2349-2988


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Isolation and Characterization of Disease Resistant Cell Lines of Cleome viscosa Using Fungal Elicitor



Address:   Amutha K., Rajagopal K., Senthil Kumaran B. and Karthikeyan S.
Department of Biotechnology, School of Life Sciences, VELS University, Velan Nagar, Pallavaram, Chennai- 600 0117, Tamil Nadu, India
*Corresponding Author:
DOI No:

ABSTRACT:
Two independent experiments were performed to isolate diseases cell lines of Cleome viscosa from leaf segments. In the first experiment, callus induction was obtained on Murashige and Skoog medium with different concentrations of Thidiazuron (TDZ). Best dedifferentiation rates of 2.08 g fresh weight occurred in the presence of 2.0 mg/l. In the second experiment, Aspergillus nigar and Aspergillus flavus were prominently isolated from garden soil and infected leaves respectively. Biochemical studies of anthocyanin accumulation at the different elicitor treated callus tissue revealed isolation and characterization of diseases resistant cell lines. Among different concentration, 0.5 gm/l mycelia of both fungal strains elucitate the maximam accumulation of anthocyanin. The best accumulation of anthocyanin was achieved with elicitors of A. flavus. Hence, pathogenic fungi are more effective for elicitation and to develop diseases resistant traits.
KEYWORDS:
callus, Cleome viscosa, anthocyanin, Aspergillus, medicinal plant
Cite:
Amutha K., Rajagopal K., Senthil Kumaran B. , Karthikeyan S.. Isolation and Characterization of Disease Resistant Cell Lines of Cleome viscosa Using Fungal Elicitor. Research J. Science and Tech. 2010; 2(4): 75-77 .
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