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Research Journal of Science and Technology
ISSN: 2349-2988(Online), 0975-4393(Print)
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Isolation and Biochemical Characterization of Bacteria Present In Roots of Water Hyacinth (Eichhornia crassipes (Mart) Solm.) In Robertson Lake Jabalpur.
P. K. Singhal
Sadhana Jaiswal1 and P. K. Singhal2
1School of Studies in Life Sciences, Pt. Ravi Shankar Shukla University Raipur, (C G) 492010 India
2Department of Biological Sciences, Rani Durgawati University, Jabalpur, India.
The present study was conducted to isolate bacteria from root of Eichhornia crassipes (water hyacinth). Bacteria were isolated in tryphtone glucose yeast extract agar (TGYEA) medium during different phases of growth of water hyacinth in Robertson lake and on basis of biochemical characteristics probable identification was done by using a PIB program, given by Bryant (1989). A total of 30 bacteria were isolated from roots of the growing water hyacinth plants in the Robertson lake Jabalpur. Out of 30 bacteria, 25 were gram - negative rods, comprising of 23 fermentative and 2 non - fermentative, 3 were gram - positive rods, and 2 were gram - positive cocci. Most of the bacteria were able to hydrolyze starch and glucose, and reduce NO3 to NO2, indicating their active role in transformation of C and N. The presence of gelatinase enzyme in most of the bacteria has indicated their role in active degradation of organic N.
Water hyacinth, bacteria, aquatic ecosystem, Robertson Lake, C and N transformation.
Sadhana Jaiswal, P. K. Singhal. Isolation and Biochemical Characterization of Bacteria Present In Roots of Water Hyacinth (Eichhornia crassipes (Mart) Solm.) In Robertson Lake Jabalpur. Research J. Science and Tech. 2011; 3(3): 164-168 .
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