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Research Journal of Science and Technology
ISSN: 2349-2988(Online), 0975-4393(Print)
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Rice Chaff a Novel Substrate for Fibrinolytic Enzyme Production by Solid State Fermentation Using Penicillium chrysogenum SGAD12
Suneetha T.B. Ashwini Patil G.M
S.M. Gopinath*, Suneetha T.B. Ashwini Patil G.M
Department of Biotechnology, Acharya Institute of Technology, Soldevanhalli, Bengaluru-560090, Karnataka, India
Rice chaff - a polished substance extracted from Oryza sativa L. cv. Devamallige was used as a novel substrate for the production of the fibrinolytic enzyme. This vital enzyme is used in thrombolytic therapy, as a clot buster. The production was done by solid state fermentation of rice chaff by Penicillium chrysogenum SGAD12, locally isolated from vegetable markets. Of the 28 strains isolated and screened, Penicillium chrysogenum SGAD12 was found to give an inhibition zone greater than 2 mm. Hence it was identified as the potential organism showing maximum fibrinolytic activity under specified culture conditions. Activity optimization was done under the parameters: Time, Inoculum ratio, Moisture content, Substrate particle size. The fibrinolytic activity was favourably maximized at 104 hrs, 7% (v/v) inoculum ratio, 35-45 % (v/w) moisture content and 500µm particle size.
Rice chaff, Fibrinolytic enzyme, Solid state fermentation, Penicillium chrysogenum SGAD12.
S.M. Gopinath, Suneetha T.B. Ashwini Patil G.M. Rice Chaff a Novel Substrate for Fibrinolytic Enzyme Production by Solid State Fermentation Using Penicillium chrysogenum SGAD12. Research J. Science and Tech. 2011; 3(5): 261-263 .
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