Volume No. :   4

Issue No. :  4

Year :  2012

ISSN Print :  0975-4393

ISSN Online :  2349-2988


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Continuous Culture of Acacia mangium Willd.



Address:   Afaque Quraishi*
School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur, India
*Corresponding Author
DOI No:

ABSTRACT:
Acacia mangium, is a fast growing leguminous tree. It forms hybrids with A. auriculiformis, by air pollination; thus, micropropagation may provide genetically pure quality planting material. Therefore in vitro cultures were initiated from nodal explants of 1-year-old Mangium plant, on Driver and Kuniyuki medium with 6-benzyl adenine and polyvinyl pyrrolidone. Shoot cultures were further proliferated using on very low level of 6-benzyl adenine. Different adjuvant were tested for shoot multiplication. Shoot cultures were maintained up to 1 ½ years with retaining the shoot proliferation capacity, by normal subculture cycles. These 1 ½ years old shoot cultures were successfully rooted in vitro on half strength medium in presence of auxin, to regenerate complete plants.
KEYWORDS:
Micropropagation, DKW medium, propagule proliferation, adjuvant, subculture, rooting
Cite:
Afaque Quraishi. Continuous Culture of Acacia mangium Willd. Research J. Science and Tech. 2012; 4(4): 168-171.
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