Volume No. :   4

Issue No. :  4

Year :  2012

ISSN Print :  0975-4393

ISSN Online :  2349-2988


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Continuous Culture of Acacia mangium Willd.

Address:   Afaque Quraishi*
School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur, India
*Corresponding Author

Acacia mangium, is a fast growing leguminous tree. It forms hybrids with A. auriculiformis, by air pollination; thus, micropropagation may provide genetically pure quality planting material. Therefore in vitro cultures were initiated from nodal explants of 1-year-old Mangium plant, on Driver and Kuniyuki medium with 6-benzyl adenine and polyvinyl pyrrolidone. Shoot cultures were further proliferated using on very low level of 6-benzyl adenine. Different adjuvant were tested for shoot multiplication. Shoot cultures were maintained up to 1 ½ years with retaining the shoot proliferation capacity, by normal subculture cycles. These 1 ½ years old shoot cultures were successfully rooted in vitro on half strength medium in presence of auxin, to regenerate complete plants.
Micropropagation, DKW medium, propagule proliferation, adjuvant, subculture, rooting
Afaque Quraishi. Continuous Culture of Acacia mangium Willd. Research J. Science and Tech. 2012; 4(4): 168-171.
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