Author(s):
Dattatraya B Thorat, Sandeep Narwane, Rahul Kunkulol, Sanjay B Bhawar
Email(s):
dattatraya_thorat@rediffmail.com
DOI:
10.52711/2349-2988.2025.00020 
Address:
Dattatraya B Thorat1*, Sandeep Narwane2, Rahul Kunkulol3, Sanjay B Bhawar4
1Pravara Rural Education Society, Institute of Pharmacy, Loni, Tal - Rahata, Dist - Ahmednagar.
2Institute of Medical Sciences (Deemed University), Loni, Tal - Rahata, Dist - Ahmednagar.
3Pravara Institute of Medical Sciences (Deemed University), Loni, Tal - Rahata, Dist - Ahmednagar.
4Pravara Rural College of Pharmacy, Loni, Tal Rahata, Dist-Ahmednagar.
*Corresponding Author
Published In:
Volume - 17,
Issue - 2,
Year - 2025
ABSTRACT:
Herbal medicine is making a dramatic comeback as the side effects of synthetic medicine are daunting and the therapeutic approach is drifting towards substituent medicine. Quercetin is a plant pigment of flavonoid. It's found in many plants and foods, such as red wine, onions, green tea, apples, and berries. Quercetin has antioxidant and anti-inflammatory effects that might help reduce swelling, kill cancer cells, control blood sugar, and help prevent heart disease. Hibiscus cannabinus Linn., Leaves (Malvaceae) is an endangered plant species occurring in the Ahmednagar district (Maharashtra), India. Leaves are used in the treatment of antibilious, antioxidant, aphrodisiac, poultice and purgative. Fraction of hydroalcoholic extract of Hibiscus cannabinus Linn., Leaves by column chromatography was done. During the column elution process, the fractions 15 - 30 have a single banding pattern which was confirmed by TLC study. Phytochemical screening showed the presence of Flavonoids, Phenolic and Tannins. Physical and chemical tests of isolated compound were conducted. By using HPTLC, FTIR, 1H-NMR and GC-MS spectra of the isolated compound was confirmed as Quercetin.
Cite this article:
Dattatraya B Thorat, Sandeep Narwane, Rahul Kunkulol, Sanjay B Bhawar. Isolation and Characterization of Quercetin from Hydroalcoholic extract of Hibiscus cannabinus Linn Leaves. Research Journal of Science and Technology. 2025; 17(2):139-7. doi: 10.52711/2349-2988.2025.00020
Cite(Electronic):
Dattatraya B Thorat, Sandeep Narwane, Rahul Kunkulol, Sanjay B Bhawar. Isolation and Characterization of Quercetin from Hydroalcoholic extract of Hibiscus cannabinus Linn Leaves. Research Journal of Science and Technology. 2025; 17(2):139-7. doi: 10.52711/2349-2988.2025.00020 Available on: https://rjstonline.com/AbstractView.aspx?PID=2025-17-2-7
REFERENCES:
1. WHO/ SARS: clinical trials on treatment using a combination of traditional Chinese medicine and western medicine, 2003. pp. 53-6.
2. WHO/ traditional medicine strategy, 2002; 2002–20052.
3. Zamiska N. et al On the trail of ancient cures Wall Street Journal, Novartis, 2006, B1, B12.
4. Eyes traditional Chinese medicine, United Press International. Available from: 2007.
5. University of Maryland Medical Center: 2013.
6. Bugyei K A, Boye G L, Addy M E, et al Clinical efficacy of a tea-bag formulation of Cryptolepis sanguinolenta root in the treatment of acute uncomplicated falciparum malaria Ghana, Med J. 2010;44(1): 3-9.
7. Firenzuoli F, Gori L, Crupi A, Neri D. et al Flavonoids: risks or therapeutic opportunities? Recenti Prog Med. 2004; 95: 345–351.
8. Trivedi P.C. Medicinal Plants: Traditional Knowledge: I. K. International Publishing House Pvt. Ltd., New Delhi. 2006; 273.
9. Williamson E M. et al Synergy and other interactions in phytomedicine. 2001; 8: 401-409.
10. Bodekar G, Mumford G. (Eds). et al Traditional, Complementary and Alternative Medicine: Policy and Public Health Perspectives. Imperial College Press, London, UK. 2007.
11. Mosihuzzaman M, Choudhary M I, et al Protocols on safety, efficacy, standardization and documentation of herbal medicine (IUPAC Technical Report). Pure and Applied Chemistry, 2008; 80:2195-2230.
12. ICDRA. 199. 6th International Conference on Drug Regulatory Authorities. WHO, Ottawa.
13. TGA, Therapeutic Goods Administration (Australia), 2004.
14. Wagner H. et al Trends and challenges in phytomedicine: research in the new millennium, In Handbook of Medicinal Plants. Yaniv Z, Bachrach U. (Eds). Haworth Medical Press, 2001; 3-28.
15. A. J. Stewart, S. Bozonnet, W. Mullen, G. I. Jenkins, M. E. Lean, and A. Crozier, et al “Occurrence of flavonols in tomatoes and tomato-based products,” Journal of Agricultural and Food Chemistry, 2000: 48 (7), 2663–2669.
16. Bohm, B. Introduction of Flavonoids; Harwood Academic Publishers: Singapore, 1998.
17. E. H. Kelly, R. T. Anthony, and J. B. Dennis, et al “Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships,” Journal of Nutritional Biochemistry, 2002:13(10), 572–584.
18. Han X., Shen T. and Lou H. et al Dietary polyphenols and their biological significance. Int J Mol Sci, 2007:9, 950-988.
19. J B Harborne. Et al Phytochemical methods, A guide to modern techniques of plant analysis. Third edition. 1998;40-44,73-79.
20. Khandelwal K R. 2006; Practical Pharmacognosy Techniques and Experiments, 15thED, Nirali Prakashan, India.
21. Kokate C C, Purohit A P and Gokhale S B. 2001; Text Book of Pharmacognosy, 7th ED, Nirali Prakashan, India.
22. M. Lopez, F. Martinez, C. Del Valle, C. Orte, and M. Miro, et al. Analysis of phenolic constituents of biological interest in red wines by high performance liquid chromatography. Journal of Chromatography A. 2001; 922(1-2): 359–363,.
23. Maria Kratchanova, Petko Denev, Milan Ciz, Antonin Lojek and Atanas Mihailov, et al Evaluation of antioxidant activity of medicinal plants containing polyphenol compounds. Comparison of two extraction systems. ACTA Biochimica polonica. 2010; 57(2): 229–234.
24. Middeltone H. 1956; Systematic Qualitative Analysis. Edward Arnnold Publishers Ltd., London.