Author(s): Karthikeyan Subbarayan, Rajagopal Kalyanaraman, Shobana Vijayanand

Email(s): karthisubbarayan@gmail.com

DOI: Not Available

Address: Karthikeyan Subbarayan, Rajagopal Kalyanaraman and Shobana Vijayanand
Dept. of Biotechnology, School of Life Sciences, VELS University, Velan Nagar, Pallavaram, Chennai- 600 0117, Tamil Nadu, India
*Corresponding Author:

Published In:   Volume - 2,      Issue - 3,     Year - 2010


ABSTRACT:
The carbon source in plant cell suspension culture is a very important factor for growth and development. In the present investigation the role of different carbon sources such as analytical grade sucrose (Hi-Media, India), commercial grade sugar, sugarcubes and jaggery (both purchased from local market) were studied in cell growth of Momordica charantia in an effort to reduce the cost instead of using high cost carbon source. Studies have revealed that significant improvement was observed in cell growth on a medium containing sugarcubes. More callus could be induced successfully from leaf and shoot tissues when cultured on MS medium supplemented with two combinations of growth regulators influence (Kin+IAA and BA+IAA). More fresh weight and the best friability of callus was obtained from leaf explants on MS medium containing 1.0 mgl-1 IAA and 0.5 mgl-1 Kin with semi-solid medium (0.6% agar). The cell suspension culture promoted the best cell growth in medium containing sugarcubes with biomass of 8.245 g fresh weight and 0.298 g dry weight in 24 days of inoculation. This is the first report using four different carbon sources in plant cell suspension cultures. The results showed that out of four carbon sources tested, sugarcubes could be the best source of carbon and it potentially reduces the cost of using costly carbon sources in cell suspension cultures.


Cite this article:
Karthikeyan Subbarayan, Rajagopal Kalyanaraman , Shobana Vijayanand. Low-Cost Alternatives for Accelerating the Plant Cell Suspension Culture of Momordica charantia L. Research J. Science and Tech. 2010; 2(3): 60-63 .


Recomonded Articles:

Author(s): S.Sharma, Vikas Kumar Jain

DOI: 10.5958/2349-2988.2015.00012.1         Access: Open Access Read More

Author(s): Adebayo, F.O., Obiekezie, S.O.

DOI: 10.5958/2349-2988.2018.00005.0         Access: Open Access Read More

Author(s): Shahanshah Gulpham

DOI:         Access: Open Access Read More

Author(s): R W Gaikwad

DOI:         Access: Open Access Read More

Author(s): M. Vijaya Sekhar Reddy, K. Sasi, K. Ashalatha, M. Madhuri

DOI: 10.5958/2349-2988.2017.00056.0         Access: Open Access Read More

Author(s): Limbesh B. Aal, Jignesh N. Parmar, Vishvesh R. Patel , Dhrubo Jyoti Sen

DOI:         Access: Open Access Read More

Author(s): Ezenobi U.V, F.J. Amaku , Agbidi, Chioma

DOI:         Access: Open Access Read More

Author(s): Siddamsetty Upendra, Siddamsetty Mahendra, Poli Sivasankar Rao

DOI: 10.5958/2349-2988.2017.00091.2         Access: Open Access Read More

Author(s): Sachin S. Rajmane, Sushil R. Mathpati, Jairaj K. Dawle

DOI: 10.5958/2349-2988.2016.00026.7         Access: Open Access Read More

Author(s): Shashikant R Pattan, Nachiket S Dighe, H V Shinde, Deepak S Musmade, Mangesh B Hole, Vinayak M Gaware

DOI:         Access: Open Access Read More

Author(s): Karunakar Shukla, PK Dubey

DOI:         Access: Open Access Read More

Author(s): Mahalakshmi. R, Saranya. R

DOI: 10.5958/2349-2988.2017.00049.3         Access: Open Access Read More

Author(s): Ravi Kumar, Swati Patil, MB Patil, Mahesh S Paschapur, Sachin R Patil

DOI:         Access: Open Access Read More

Author(s): Sachin Kumar, Pratishtha Gupta

DOI: 10.5958/2349-2988.2016.00008.5         Access: Open Access Read More

Author(s): Raghav M. , Dixit S.

DOI:         Access: Open Access Read More

Research Journal of Science and Technology (RJST) is an international, peer-reviewed journal, devoted to science and technology...... Read more >>>

RNI: Not Available                     
DOI: 10.5958/2349-2988 


Recent Articles




Tags