The present investigation includes the preliminary screening of phytochemicals and evaluation of an in vitro antidiabetic activity of Azima tetracantha LAM extracts. Plant material was sub¬jected to the extract preparation by soxhlet apparatus by using various solvents such as aqueous, ethanol, chloroform, petroleum ether. The various kinds of phytochemicals were detected and then in vitro antidiabetic activity of Azima tetracantha LAM ethanol and aqueous extracts were detected by using alpha amy¬lase and alpha glucosidase enzyme in an in vitro model. The study reveals the presence of phytochemicals such as carbohydrate, tannin, flavonoids, phenols etc., Among the various extracts the aqueous and ethanolic extracts which contains the large number of phytoconstituents. The Azima tetracantha LAM extracts has been successfully inhibited both the enzymes in an in vitro model. The aqueous extracts of Azima tetracantha LAM inhibited the alpha amylase and alpha glucosidase enzymes as 48.59% and 64.99% respectively. The ethanolic extract of Azima tetracantha LAM inhibited the alpha amylase and alpha glucosidase enzymes as 65.34% and 58.48% respectively. From the results obtained in the current studies, the Azima tetracantha LAM extracts have a prominent antidiabetic property in an in vitro model and further studies can be carried out in an in vivo model and the isolation of active compound from Azima tetracantha LAM extract is needed.
Cite this article:
B. Edwin Jose, P. Panneerselvam. In Vitro Antidiabetic activity of Ethanol and Aqueous leaf extracts of Azima tetracantha Lam. Research J. Science and Tech. 2019; 11(1):64-68. doi: 10.5958/2349-2988.2019.00009.3