Author(s):
M. K. Ranganath, Prasanta Deka, Kalyani Arikatla
Email(s):
ranga9rpc@gmail.com , prasantadeka00@gmail.com , arikatlakalyani707@gmail.com
DOI:
10.5958/2349-2988.2020.00025.X 
Address:
M. K. Ranganath, Prasanta Deka*, Kalyani Arikatla
Department of Pharmaceutical Analysis, Krupanidhi College of Pharmacy, Bangalore-560035.
*Corresponding Author
Published In:
Volume - 12,
Issue - 3,
Year - 2020
ABSTRACT:
The present work describes a simple, rapid and reproducible reverse phase high performance liquid chromatography also termed as RP-HPLC method for simultaneous estimation of amlodipine and valsartan. C18 column having 150 x 4.6mm diameter was used as stationary phase and a mobile phase containing HPLC grade methanol and water in the ratio of 90; 10 v/v respectively. Orthophosphoric acid used for the pH adjustment to 3.2. The flow rate was 1.0 ml/min and elutes were detected by UV detector at 254nm. The retention time were found to be 1.1 min and 2.1 min for amlodipine and valsartan respectively. The developed method was validated according to the ICH guidelines and found to be linear within the range of 8-28mcg/ml for both drugs. Precision was found to be <2%. The proposed method was successfully used for the simultaneous estimation of amlodipine and valsartan in bulk and formulation. Validation studies revealed that method is specific, rapid and reliable. The % of recovery and less %RSD confirms the suitability of proposed method for routine analysis.
Cite this article:
M. K. Ranganath, Prasanta Deka, Kalyani Arikatla. Simultaneous method development and Validation of Amlodipine and Valsartan by HPLC. Research J. Science and Tech. 2020; 12(3):183-189. doi: 10.5958/2349-2988.2020.00025.X
Cite(Electronic):
M. K. Ranganath, Prasanta Deka, Kalyani Arikatla. Simultaneous method development and Validation of Amlodipine and Valsartan by HPLC. Research J. Science and Tech. 2020; 12(3):183-189. doi: 10.5958/2349-2988.2020.00025.X Available on: https://rjstonline.com/AbstractView.aspx?PID=2020-12-3-3
REFERENCE:
1. Ashish Chauhan, Bharti Mittu and Priyanka Chauhan. Analytical method development and validation: a concise review. Journal of Analytical and Bioanalytical Techniques. 2015; 6(1): 1-5
2. Azim Md. Sabir, Mitra Moloy, Bhasin Parmindar S. HPLC method development and validation: a review. international research journal of pharmacy. 2013; 4(4): 39-46
3. S. B. Wankhede, S. B. Wadkar, K. C. Raka and S. S. Chitlange. Simultaneous estimation of amlodipine besilate and Olmesartan medoxomil in pharmaceutical dosage form. International journal of pharmaceutical sciences. 2009; 71(5): 563-567
4. P.S. Jain, M.K. Patel, A P Gorle, A J Chaudhari and S J Surana. Stability indicating method for the simultaneous estimation of Olmesartan medoxomil, amlodipine besylate and hydrochlorothiazide by RP-HPLC method in tablet dosage form. Journal of chromatographic science. 2012; 50: 680-687
5. shah SK, Asnani AJ, Kawade DP, Dangre SC, Arora SK, Yende SR. simultaneous quantitative analysis of Olmesartan medoxomil and amlodipine besylate in plasma by high performance liquid chromatography technique. Journal of young pharmacists. 2012; 4(2): 88-94
6. Silvana E. Vignaduzzo, Patricia M. Castellano a and Teodoro S. Kaufman. Development and validation of an HPLC method for the simultaneous determination of amlodipine, hydrochlorothiazide, and valsartan in tablets of their novel triple combination and binary pharmaceutical associations. Journal of Liquid Chromatography and Related Technologies. 2011; 34(19): 34-19
7. Rasha A. Shaalan a and Tarek S. Belal. gradient HPLC-DAD determination of the antihypertensive mixture of amlodipine besylate, valsartan, and hydrochlorothiazide in combined pharmaceutical tablets. Journal of Liquid Chromatography and Related Technologies. 2012; 35(2): 215-230
8. Mustafa Celebier, Mustafa Sinan Kaynak, Sacide Altınoz1, Selma Sahin. HPLC method development for the simultaneous analysis of amlodipine and valsartan in combined dosage forms and in vitro dissolution studies. Brazilian Journal of Pharmaceutical Sciences. 2010; 46(4): 761-768
9. Nesrin K. Ramadan a, Heba M. Mohamed a and Azza A. Moustafa. Rapid and Highly Sensitive HPLC and TLC Methods for Quantitation of Amlodipine Besilate and Valsartan in Bulk Powder and in Pharmaceutical Dosage Forms and in Human Plasma. Analytical Letters. 2010; 43(4): 570-581
10. Nitesh Maheshwari. Validated reverse phase high performance liquid chromatography method for simultaneous estimation of valsartan potassium and amlodipine besylate in tablet dosage form. International journal of drug development and research. 2013; 5(3): 237-244
11. Asmita Y. Kamble, Mahadeo V. Mahadik, Laxman D. Khatal and Sunil R. Dhaneshwar. Validated HPLC and HPTLC method for simultaneous quantitation of amlodipine besylate and Olmesartan medoxomil in bulk drug and formulation. Analytical Letters. 2010; 43(2): 251-258
12. Susheel John Varghese a and Thengungal Kochupappy Ravi. Quantitative simultaneous determination of amlodipine, valsartan, and hydrochlorothiazide in "Exforge HCT" tablets using high-performance liquid chromatography and high-performance thin-layer chromatography. Journal of Liquid Chromatography and Related Technologies. 2011; 34(12): 981-994
13. Swamy, Ravindra, Sowmya. A new RP-HPLC method development and validation for the simultaneous estimation of amlodipine and valsartan in tablet dosage forms. Asian journal of pharmaceutical analysis. AJP Ana. 2014; 4(3): 103-07
14. Koralla, Konidala, Rao, Begum. Stability indicating RP-HPLC method for simultaneous estimation of ramipril and amlodipine besylate in pharmaceutical dosage form. Asian journal of pharmaceutical research. AJP Ana. 2016; 6(4): 142-49