Volume No. :   3

Issue No. :  2

Year :  2011

ISSN Print :  0975-4393

ISSN Online :  2349-2988


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Caco-2 cell – A new vision in cell world

Address:   Yatri R. Shah1, Parimal M. Prajapati2, Ravi N. Patel3, Urvi Y. Patel3 and Dhrubo Jyoti Sen3
1Shree H. N. Shukla Institute of Pharmaceutical Education & Research, Gujarat Technological University, Behind Marketing Yard, Nr. Lalpari Lake, Amargadh (Bhichari), Rajkot, Gujarat
2I. K. Patel College of Pharmaceutical Education and Research, Samarth Campus, Opp. Sabar Dairy, Himmatnagar-383001, Sabarkantha, Gujarat
3Department of Pharmaceutical Chemistry, Shri Sarvajanik Pharmacy College, Hemchandracharya North Gujarat University, Arvind Baug, Mehsana-384001, Gujarat, India

Caco-2 cells are most commonly used not as individual cells, but as a confluent monolayer on a cell culture insert filter (e.g., Transwell). When cultured in this format, the cells differentiate to form a polarized epithelial cell monolayer that provides a physical and biochemical barrier to the passage of ions and small molecules. The Caco-2 monolayer is widely used across the pharmaceutical industry as an in-vitro model of the human small intestinal mucosa to predict the absorption of orally administered drugs. The correlation between the in vitro apparent permeability (P¬app) across Caco-2 monolayers and the in-vivo fraction absorbed (fa) is well established.
Caco-2 cell
Yatri R. Shah, Parimal M. Prajapati, Ravi N. Patel, Urvi Y. Patel, Dhrubo Jyoti Sen. Caco-2 cell – A new vision in cell world. Research J. Science and Tech. 3(2): March-April. 2011: 51-54.
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