Yatri R. Shah, Parimal M. Prajapati, Ravi N. Patel, Urvi Y. Patel, Dhrubo Jyoti Sen
Yatri R. Shah1, Parimal M. Prajapati2, Ravi N. Patel3, Urvi Y. Patel3 and Dhrubo Jyoti Sen3
1Shree H. N. Shukla Institute of Pharmaceutical Education & Research, Gujarat Technological University, Behind Marketing Yard, Nr. Lalpari Lake, Amargadh (Bhichari), Rajkot, Gujarat
2I. K. Patel College of Pharmaceutical Education and Research, Samarth Campus, Opp. Sabar Dairy, Himmatnagar-383001, Sabarkantha, Gujarat
3Department of Pharmaceutical Chemistry, Shri Sarvajanik Pharmacy College, Hemchandracharya North Gujarat University, Arvind Baug, Mehsana-384001, Gujarat, India
Volume - 3,
Issue - 2,
Year - 2011
Caco-2 cells are most commonly used not as individual cells, but as a confluent monolayer on a cell culture insert filter (e.g., Transwell). When cultured in this format, the cells differentiate to form a polarized epithelial cell monolayer that provides a physical and biochemical barrier to the passage of ions and small molecules. The Caco-2 monolayer is widely used across the pharmaceutical industry as an in-vitro model of the human small intestinal mucosa to predict the absorption of orally administered drugs. The correlation between the in vitro apparent permeability (P¬app) across Caco-2 monolayers and the in-vivo fraction absorbed (fa) is well established.
Cite this article:
Yatri R. Shah, Parimal M. Prajapati, Ravi N. Patel, Urvi Y. Patel, Dhrubo Jyoti Sen. Caco-2 cell – A new vision in cell world. Research J. Science and Tech. 3(2): March-April. 2011: 51-54.